MORNING SPEAKERS

·         Organization of the Hematopoietic Environment in the Bone Marrow – Leslie Silberstein, MD, Director, Joint Program in Transfusion Medicine, Children's Hospital Boston, Dana-Farber Cancer Institute, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 

·         How Imaging Cytometry Makes a Difference in Answering Complex Questions – Stephan Ruetz, PhD, Laboratory Head, Oncology Research, Novartis Institute for Biomedical Research, Basel, Switzerland 

·         Mechanisms of Heart Muscle Cell Proliferation – Bernhard Kühn, MD, Associate in Cardiology, Children’s Hospital Boston, and Assistant Professor of Pediatrics, Harvard Medical School, Boston MA 

·         Laser Scanning Cytometry for Assessment of DNA Damage – Zbigniew Darzynkiewicz, MD, PhD, Director, Brander Cancer Research Institute, Valhalla, NY 

·         Novel Cell-Array Technology Combined with the Power of Laser Scanning Cytometry (LSC) in High-Throughput DNA Content and FISH Analysis – Kohsuke Sasaki, MD, Professor of Pathology and Director of the Department of Pathology, Yamaguchi University Graduate School of Medicine, UBbe, Yamaguchi, Japan 

·         Safety and Efficacy of Islet Cell Transplantation – Fouad Kandeel, MD, PhD, Director, Division of Diabetes, Endocrinology and Metabolism, City of Hope Medical Center, Duarte, CA  

·         Preclinical Pharmacokinetic and Biomarker Analysis of a Small Molecule Inhibitor of Aurora Kinases in Human Xenograft Tumor and Surrogate Tissues – Gloria Juan, PhD, Principal Scientist, Clinical Immunology, Amgen, Thousand Oaks, CA

·         Cell Surface Immunophenotyping by LSC: History, Present, and Future – Richard Clatch, MD, PhD, Chair, Department of Pathology, Lake Forest Hospital, Lake Forest, IL

·         Additional speakers to be announced

AFTERNOON WORKSHOPS

Track 1:  Designing and Implementing QIC Experiments

This track will provide researchers with an introduction to QIC technology and its most common applications.  The workshops in this track will provide a solid background in the technology for those attendees without an in-depth familiarity with the technology.  Topics will include quantitative and imaging end-points, segmentation strategies, basic applications, and data analysis options.                    

Day 1 – Designing QIC experiments: Experiment design and setup, basic instrument operation, multicolor analysis, and specialized algorithms for complex analysis – Instructor: William Telford, NIH

Day 2 – Cellular applications: DNA content/cell cycle/apoptosis/ multiplexing/ high content and high throughput – Instructor: Heather Zecchini, Cancer Research UK.

Day 3 – Tissue Applications: Chromatic Analysis/ Fluorescent analysis/ Fluorochromatic analysis – Instructor: David Krull - GSK

Track 2:  Advanced QIC Workshops

Designed for more experienced QIC users, this track is structured to foster communications around a particular topic among researchers currently using the technology, so that the skills and knowledge of each of the participants can provide insight to others.  Led by a facilitator, discussions will be focused on a particular application area and will include protocol and application presentations from participants in order to provide a productive discussion of the whys and hows of specific solutions.  Topics may include quantitative in situ protein expression analysis, cell signaling pathways, cell cycle/DNA damage/apoptosis analysis, live-cell toxicologic assays, and advanced segmentation strategies.

Day 1 – In Situ Protein Expression Analysis in tissue sections and tissue microarrays, Facilitator: Sue Ludmann, Amgen. Panelists: David Krull, GSK; Ivan Todorov, City of Hope Medical Center.

Day 2 – Advanced Cell Cycle/ DNA Damage/Cell signaling, Facilitator: Stephan Ruetz, Novartis. Panelists: James Jacobberger, Case Western Reserve University; Zbigniew Darzynkiewicz, Brander Cancer Research Insitute.

Day 3 – New developments: Platelet Production in the Human Vasculature, Live Cell Analysis, Advanced Image Analysis, Facilitator: Jonathon Thon, Children's Hospital Boston.

Track 3:  Custom application development and analysis (2 locations)

Designed for attendees with specific projects, this track will provide the opportunity for personal consultation, scanning, and analysis with the CompuCyte scientific staff. 

This track is open only to attendees from Harvard-affiliated institutions. Full symposium registration and sample submission are required.